Mouse BAFFR/TNFRSF13C Protein (BAF-MM10R)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
  • Amino Acid Range: Ser10-Ala71
Catalog #BAF-MM10R
Regular price $346.50
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Mouse BAFFR/TNFRSF13C Protein (BAF-MM10R) Summary

Product Description

Recombinant Mouse BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser10-Ala71.

Product Background

BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse BAFFR/TNFRSF13C Protein (BAF-MM10R) Specifications/Details

Protein
BAFFR/TNFRSF13C
Synonyms
CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ser10-Ala71
Molecular Weight
The protein has a predicted MW of 7.79 kDa. Due to glycosylation, the protein migrates to 18-28 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Mouse BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse BAFFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse BAFFR, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 2.56 nM as determined in BLI assay (Gator® Prime). Loaded Mouse BAFFR, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 2.56 nM as determined in BLI assay (Gator® Prime).
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