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Mouse BCHE/Butyrylcholinesterase Protein (BCE-MM101) Summary
Product Description |
Recombinant Mouse BCHE/Butyrylcholinesterase Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu30-Leu603. |
Product Background |
Butyrylcholinesterase is a serine hydrolase biochemically related to the cholinergic enzyme acetylcholinesterase. It is capable of hydrolyzing esters of choline. Butyrylcholinesterase has unique enzymatic properties and is widely distributed in the nervous system, raising the possibility of its involvement in neural function. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse BCHE/Butyrylcholinesterase Protein (BCE-MM101) Specifications/Details
Protein |
BCHE/Butyrylcholinesterase |
Synonyms |
Cholinesterase; BCHE; CHE1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu30-Leu603 |
Molecular Weight |
The protein has a predicted MW of 66.13 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to cleave Butyrylthiocholine. The specific activity is > 50000 pmoles/min/ug. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse BCHE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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