Product Description |
Recombinant Mouse Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Phe53-Pro994. |
Product Background |
Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites. |
Product Category |
Recombinant Protein |
Target |
Beta Klotho |
Synonyms |
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; MGC142213;βKlotho; β-klotho; klotho β like; Klotho β |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Phe53-Pro994 |
Molecular Weight |
The protein has a predicted MW of 109.91 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 36.97 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>90% as determined by Bis-Tris PAGE; >90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Beta Klotho, His Tag with an affinity constant of 36.97 nM as determined in SPR assay (Biacore T200). |
|
|
Mouse Beta Klotho on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
|
|
The purity of Mouse Beta Klotho Protein is greater than 90% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
