Mouse Cathepsin H Protein (CSH-MM101)
Mouse Cathepsin H Protein (CSH-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >700 pmol/min/ug.
  • Amino Acid Range: Ala21-Val333
Catalog #CSH-MM101
Regular price $346.50
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Size
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Mouse Cathepsin H Protein (CSH-MM101) Summary

Product Description

Recombinant Mouse Cathepsin H Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala21-Val333.

Product Background

Cathepsin H (CatH) is a lysosomal cysteine protease with a unique aminopeptidase activity that is extensively expressed in the lung, pancreas, thymus, kidney, liver, skin, and brain. Owing to its specific enzymatic activity, CatH has critical effects on the regulation of biological behaviours of cancer cells and pathological processes in brain diseases.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse Cathepsin H Protein (CSH-MM101) Specifications/Details

Protein
Cathepsin H
Synonyms
Cathepsin B3; Cathepsin BA; Ctsh
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ala21-Val333
Molecular Weight
The protein has a predicted MW of 36.23 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >700 pmol/min/ug.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse Cathepsin H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Cathepsin H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Cathepsin H is greater than 95% as determined by SEC-HPLC. The purity of Mouse Cathepsin H is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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