Product Description |
Recombinant Mouse CD10/MME Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr52-Trp750. |
Product Background |
CD10 is an endopeptidase that degrades various bioactive peptides in the extracellular matrix. In addition to enzymatic degradation, it affects multiple intracellular signal transduction pathways. CD10 expression has been extensively studied in human epithelial cancers of numerous organs and sites. CD10 expression pattern depended on the histotypes of thyroid lesions. When possible metastatic tumours and non-epithelial tumours are excluded, high CD10 expression may be useful in determining whether a primary thyroid carcinoma includes an anaplastic component. |
Product Category |
Recombinant Protein |
Target |
CD10/MME |
Synonyms |
Neprilysin; Atriopeptidase; Enkephalinase; NEP; Neutral; SFE; CD10; MME; CALLA; DKFZp686O16152; MGC126681; MGC126707 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Tyr52-Trp750 |
Molecular Weight |
The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 82-110 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse CD10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse CD10 is greater than 95% as determined by SEC-HPLC. |
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