Mouse CD13/ANPEP Protein, Ultra Low Endotoxin (ANP-MM101-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >1,000-pmol/min/µg.
  • Amino Acid Range: Lys69-Ser966
Catalog #ANP-MM101-UL
Regular price $327.00
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Mouse CD13/ANPEP Protein, Ultra Low Endotoxin (ANP-MM101-UL) Summary

Product Description

Recombinant Mouse CD13/ANPEP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys69-Ser966.

Product Background

CD13/aminopeptidase N is a widely expressed ectoenzyme with multiple functions. As an enzyme, CD13 regulates activities of numerous cytokines by cleaving their N-terminals and is involved in Ag processing by trimming the peptides bound to MHC class II. Independent of its enzymatic activity, cell membrane CD13 functions by cross-linking-induced signal transduction, regulation of receptor recycling, enhancement of FcγR-mediated phagocytosis, and acting as a receptor for cytokines.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse CD13/ANPEP Protein, Ultra Low Endotoxin (ANP-MM101-UL) Specifications/Details

Protein
CD13/ANPEP
Synonyms
AP-N; AP-M; ANPEP; APN; CD13; PEPN; Cd13; CD13APN; gp150; LAP1; PEPNhAPN
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Lys69-Ser966
Molecular Weight
The protein has a predicted MW of 103.9 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >1,000-pmol/min/µg.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CD13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD13 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD13 is greater than 95% as determined by SEC-HPLC.
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