SPR equilibrium binding curve showing response units plotted against analyte concentration, used to determine binding affinity of recombinant protein interactions
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test). The affinity constant of 4.00 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Asp29-Leu348
Catalog #CD1-MM455
Regular price $346.50
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Mouse CD155/PVR Protein (CD1-MM455) Summary

Product Description

Recombinant Mouse CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp29-Leu348.

Product Background

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Mouse CD155/PVR Protein (CD1-MM455) Specifications/Details

Protein
CD155/PVR
Synonyms
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Asp29-Leu348
Molecular Weight
The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test). The affinity constant of 4.00 uM as determined in SPR assay (Biacore T200).
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 uM as determined in SPR assay (Biacore T200). Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test). Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test).
Mouse CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay . Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay .
The purity of Mouse CD155 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD155 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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