Mouse CD155/PVR Protein (CD1-MM455)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test). The affinity constant of 4.00 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp29-Leu348
Catalog #CD1-MM455
Regular price $315.00
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Product Description
Recombinant Mouse CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp29-Leu348.
Product Background
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
Product Category
Recombinant Protein
Target
CD155/PVR
Synonyms
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Asp29-Leu348
Molecular Weight
The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test). The affinity constant of 4.00 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test).
Mouse CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD155 is greater than 95% as determined by SEC-HPLC.

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