SPR sensorgram showing the binding interaction between immobilized mouse CD200 receptor 1 (His tag) and mouse CD200 (His tag), with response units (RU) plotted against time to illustrate binding kinetics and dissociation phases at various analyte concentrations.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.19 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln31-Gly232
Catalog #CD2-MM120
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Size
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Mouse CD200/OX-2 Protein (CD2-MM120) Summary

Product Description

 Recombinant Mouse CD200/OX-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln31-Gly232.

Product Background

 CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modulation of CD200 receptors was shown to improve clinical and inflammatory outcomes of preclinical asthma models.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse CD200/OX-2 Protein (CD2-MM120) Specifications/Details

Protein
 CD200/OX-2
Synonyms
 CD200; MOX1; MOX2; My033; MRC; OX-2; OX2
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gln31-Gly232
Molecular Weight
 The protein has a predicted MW of 23.6 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 1.19 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CD200 R1, His Tag immobilized on CM5 Chip can bind Mouse CD200, His Tag with an affinity constant of 1.19 uM as determined in SPR assay (Biacore T200). Mouse CD200 R1, His Tag immobilized on CM5 Chip can bind Mouse CD200, His Tag with an affinity constant of 1.19 uM as determined in SPR assay (Biacore T200).
Mouse CD200 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD200 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD200 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD200 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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