Mouse CD27/TNFRSF7 Protein (CD2-MM327)

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 20.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Pro24-Arg182

Catalog: CD2-MM327

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Product Description	Recombinant Mouse CD27/TNFRSF7 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Pro24-Arg182.
Product Background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Product Category	Recombinant Protein

Target	CD27/TNFRSF7
Synonyms	CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
Accession	P41272-1
Species	Mouse
Amino Acid Range	Pro24-Arg182
Molecular Weight	The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 20.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-mFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 20.4ng/ml determined by ELISA.
	The purity of Mouse CD27 is greater than 95% as determined by SEC-HPLC.
	Mouse CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.