Mouse CD27/TNFRSF7 Protein (CD2-MM327)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD27, mFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Pro24-Arg182

Catalog: CD2-MM327

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Product Description Recombinant Mouse CD27/TNFRSF7 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Pro24-Arg182.

Product Background

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

Product Category

Recombinant Protein

Target

CD27/TNFRSF7
Synonyms CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor

Gene/Protein ID

P41272-1

Species

Mouse

Exact Sequence

Pro24-Arg182
Molecular Weight The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse CD27, mFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-mFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD27, mFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA.
The purity of Mouse CD27 is greater than 95% as determined by SEC-HPLC.
Mouse CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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