Mouse CD27/TNFRSF7 Protein (CD2-MM127)
Mouse CD27/TNFRSF7 Protein (CD2-MM127)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Pro24-Arg182
Catalog #CD2-MM127
Regular price $315.00
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Product Description
Recombinant Mouse CD27/TNFRSF7 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro24-Arg182.
Product Background
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Product Category
Recombinant Protein
Target
CD27/TNFRSF7
Synonyms
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Pro24-Arg182
Molecular Weight
The protein has a predicted MW of 19.40 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Mouse CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD27 is greater than 95% as determined by SEC-HPLC.

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