Mouse CD27/TNFRSF7 Protein (CD2-MM127)
Mouse CD27/TNFRSF7 Protein (CD2-MM127)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
  • Amino Acid Range: Pro24-Arg182
Catalog #CD2-MM127
Regular price $346.50
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Size
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Mouse CD27/TNFRSF7 Protein (CD2-MM127) Summary

Product Description

Recombinant Mouse CD27/TNFRSF7 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro24-Arg182.

Product Background

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Mouse CD27/TNFRSF7 Protein (CD2-MM127) Specifications/Details

Protein
CD27/TNFRSF7
Synonyms
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Pro24-Arg182
Molecular Weight
The protein has a predicted MW of 19.40 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. Immobilized Mouse CD27, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Mouse CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD27 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD27 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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