Regular price
$375.00
Order by 4pm ET for delivery on:
Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-MM105) Summary
Product Description |
Recombinant Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed as a heterodimer of CD3E and CD3D by co-expression in HEK293. Subunit CD3E is fused with a Flag tag at the C-terminus and subunit CD3D is fused with a His tag at the C-terminus.It contains Asp22-Asp108 (CD3E) and Phe22-Ala105 (CD3D). |
Product Background |
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-MM105) Specifications/Details
Protein |
CD3E&CD3D/CD3 epsilon&CD3 delta |
Synonyms |
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Asp22-Asp108(CD3E) & Phe22-Ala105(CD3D) |
Molecular Weight |
The protein has a predicted MW of 15.34 kDa (CD3E) and 14.81 kDa (CD3D). Due to glycosylation, the protein migrates to 18-22 kDa and 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3E Antibody (145-2C11) with the EC50 of 41.2ng/ml determined by ELISA. |
Product Tag |
C-His, C-Flag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Mouse CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3E Antibody (145-2C11) with the EC50 of 41.2ng/ml determined by ELISA. |
|
|
Mouse CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse CD3E&CD3D is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
