Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-MM205) Summary
Product Description |
Recombinant Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of CD3E and CD3D.It contains Asp23-Asp108(CD3E) and Phe22-Ala105(CD3D). |
Product Background |
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-MM205) Specifications/Details
Protein |
CD3E&CD3D/CD3 epsilon&CD3 delta |
Synonyms |
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Asp23-Asp108(CD3E) & Phe22-Ala105(CD3D) |
Molecular Weight |
The protein has a predicted MW of 36.1 kDa (CD3E) and 35.2 kDa (CD3D). Due to glycosylation,the protein migrates to 50-65 kDa and 40-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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