Name: Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140)
Brand: KactusBio
SKU: CDL-MM140-100UG
Price: 240.0 USD
Availability: InStock

Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140)

Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).

Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test).

Mouse CD40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Met112-Leu260

Catalog #CDL-MM140

Size

100UG

500UG

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Product Description	Recombinant Mouse CD40 Ligand/TNFSF5 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Met112-Leu260.
Product Background	CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	CD40 Ligand/TNFSF5
Synonyms	CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession	P27548
Species	Mouse
Biotinylated	no
Amino Acid Range	Met112-Leu260
Molecular Weight	The protein has a predicted MW of 17.50 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test).
	Mouse CD40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.