Graph showing the Mouse CD40 Ligand, His Tag ELISA with a 0.1 µg concentration of Human CD40, hFc Tag per well. The x-axis represents the log concentration of Mouse CD40 Ligand, His Tag (µg/ml), and the y-axis shows the mean absorbance (OD450). The curve indicates a dose-dependent increase in absorbance.
Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140)
Graph showing the Mouse CD40 Ligand, His Tag ELISA with a 0.1 µg concentration of Human CD40, hFc Tag per well. The x-axis represents the log concentration of Mouse CD40 Ligand, His Tag (µg/ml), and the y-axis shows the mean absorbance (OD450). The curve indicates a dose-dependent increase in absorbance.
Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met112-Leu260
Catalog #CDL-MM140
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Size
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Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140) Summary

Product Description

 Recombinant Mouse CD40 Ligand/TNFSF5 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Met112-Leu260.

Product Background

 CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140) Specifications/Details

Protein
 CD40 Ligand/TNFSF5
Synonyms
 CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Met112-Leu260
Molecular Weight
 The protein has a predicted MW of 17.50 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test). Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test).
Mouse CD40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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