Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140)
Mouse CD40 Ligand/TNFSF5 Protein (CDL-MM140)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Met112-Leu260
Catalog #CDL-MM140
Regular price $240.00
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Product Description
Recombinant Mouse CD40 Ligand/TNFSF5 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Met112-Leu260.
Product Background
CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category
Recombinant Protein
Target
CD40 Ligand/TNFSF5
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Met112-Leu260
Molecular Weight
The protein has a predicted MW of 17.50 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
N-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
Immobilized Human CD40, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 9.4ng/ml determined by ELISA (QC Test).
Mouse CD40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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