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Mouse CD40/TNFRSF5 Protein, Ultra Low Endotoxin (CD4-MM140-UL) Summary
Product Description |
Recombinant Mouse CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Arg193. |
Product Background |
CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Mouse CD40/TNFRSF5 Protein, Ultra Low Endotoxin (CD4-MM140-UL) Specifications/Details
Protein |
CD40/TNFRSF5 |
Synonyms |
CD40; CD40L receptor;- TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013; |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Val24-Arg193 |
Molecular Weight |
The protein has a predicted MW of 19.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse CD40, His Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse CD40, His Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). |
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Mouse CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Mouse CD40, His Tag on Anti-His-Biosensor can bind Mouse CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.10 nM as determined in BLI assay . |
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The purity of Mouse CD40 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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