Mouse CD83 Protein (CD8-MM283) Summary
Product Description |
Recombinant Mouse CD83 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Met22-Ala134. |
Product Background |
CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse CD83 Protein (CD8-MM283) Specifications/Details
Protein |
CD83 |
Synonyms |
CD83; HB15; BL11; CD83 molecule |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Met22-Ala134 |
Molecular Weight |
The protein has a predicted MW of 39.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse CD83 is greater than 95% as determined by SEC-HPLC. |
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