Regular price
$248.00
Order by 4pm ET for delivery on:
Mouse Coagulation factor III/Tissue Factor Protein (TFR-MM103) Summary
Product Description |
Recombinant Mouse Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala29-Glu251. |
Product Background |
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Mouse Coagulation factor III/Tissue Factor Protein (TFR-MM103) Specifications/Details
Protein |
Coagulation factor III/Tissue Factor |
Synonyms |
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala29-Glu251 |
Molecular Weight |
The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 42-55 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <5 µg/mL. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse Coagulation factor III is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
