Product Description |
Recombinant Mouse Complement C3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile25-Asn1663. |
Product Background |
Complement component 3 (C3), a pivotal molecule in the complement system, is an essential immune mediator in various diseases, including psoriasis. C3 deficiency promoted imiquimod-induced skin cell apoptosis and supported greater proportions of IFN-γ T cells in the inflamed tissues. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Complement C3 |
Synonyms |
Complement component 3;HSE-MSF;C3;CPAMD1;AHUS5;ARMD9;ASP;C3a;C3b;HEL-S-62p |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ile25-Asn1663 |
Molecular Weight |
The protein has a predicted MW of 187.86 kDa. Due to glycosylation, the protein migrates to 65-68 kDa, 110-115 kDa and 165-180 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Mouse Complement C3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse Complement C3 is greater than 95% as determined by SEC-HPLC. |
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