SEC-HPLC chromatogram of recombinant mouse DDC protein showing a sharp primary peak at 9.205 minutes with a minor preceding peak at 8.269 minutes, indicating high purity with minimal aggregation.
Mouse DDC Protein (DDC-MB101)
  • Express System: Baculovirus-Insect Cells(Sf9)
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to-3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1500-pmol/min/µg.
  • Amino Acid Range: Met1-Glu480
Catalog #DDC-MB101
Regular price $330.00
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Size
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Mouse DDC Protein (DDC-MB101) Summary

Product Description

Recombinant Mouse DDC Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the C-terminus.It contains Met1-Glu480.

Product Background

Aromatic L-amino acid decarboxylase (AADC) deficiency is a rare genetic disorder characterized by deficient synthesis of dopamine and serotonin. It presents in early infancy, and causes severe developmental disability and lifelong motor, behavioral, and autonomic symptoms including oculogyric crises (OGC), sleep disorder, and mood disturbance.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse DDC Protein (DDC-MB101) Specifications/Details

Protein
DDC
Synonyms
AADC
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Met1-Glu480
Molecular Weight
The protein has a predicted MW of 55.40 kDa same as Bis-Tris PAGE result.
Activity
Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to-3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1500-pmol/min/µg.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
Baculovirus-Insect Cells(Sf9)
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 20mM Tris, 0.3M NaCl, 10% Glycerol (pH 8.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse DDC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse DDC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse DDC is greater than 95% as determined by SEC-HPLC. The purity of Mouse DDC is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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