Mouse DR3/TNFRSF25 Protein (DR3-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln31-Met196

Catalog: DR3-MM101

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Product Description Recombinant Mouse DR3/TNFRSF25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln31-Met196.
Product Category Recombinant Protein
Target DR3/TNFRSF25
Gene/Protein ID B1AWN9
Species Mouse
Exact Sequence Gln31-Met196
Molecular Weight The protein has a predicted MW of 18.79 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
The purity of Mouse DR3 is greater than 95% as determined by SEC-HPLC.
Mouse DR3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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