Product Description |
Recombinant Mouse DR3/TNFRSF25 Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Gln31-Met196. |
Product Background |
Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death. |
Product Category |
Recombinant Protein |
Target |
DR3/TNFRSF25 |
Synonyms |
APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln31-Met196 |
Molecular Weight |
The protein has a predicted MW of 18.79 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse DR3, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8 ng/ml determined by ELISA (QC Test). The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse DR3, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8 ng/ml determined by ELISA (QC Test). |
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Mouse DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse DR3 is greater than 95% as determined by SEC-HPLC. |
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