Mouse DR3/TNFRSF25 Protein (DR3-MM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln31-Met196

Catalog: DR3-MM101

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Product Description	Recombinant Mouse DR3/TNFRSF25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln31-Met196.
Product Background	TNF-like ligand 1 A (TL1A) and death receptor 3 (DR3) are a ligand-receptor pair involved in the pathogenesis of inflammatory bowel disease. Group 3 innate lymphoid cells (ILC3s) regulate intestinal immunity and highly express DR3.
Product Category	Recombinant Protein

Target	DR3/TNFRSF25
Gene/Protein ID	B1AWN9
Species	Mouse
Exact Sequence	Gln31-Met196
Molecular Weight	The protein has a predicted MW of 18.79 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse DR3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test).
	The purity of Mouse DR3 is greater than 95% as determined by SEC-HPLC.
	Mouse DR3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.