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Mouse ECSCR Protein (ECS-MM201) Summary
Product Description |
Recombinant Mouse ECSCR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln19-Thr130. |
Product Background |
Endothelial cell-specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Mouse ECSCR Protein (ECS-MM201) Specifications/Details
Protein |
ECSCR |
Synonyms |
ARIA; ECSCR; ECSM2 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln19-Thr130 |
Molecular Weight |
The protein has a predicted MW of 38.6 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse ECSCR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse ECSCR is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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