SEC-HPLC chromatogram showing a single major protein peak at ~7.566 minutes, indicating high purity and monodispersity of the sample.
Mouse Ephrin-A3/EFNA3 Protein (EFN-MM2A3)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gln23-Gly206
Catalog #EFN-MM2A3
Regular price $132.00
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Size
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Mouse Ephrin-A3/EFNA3 Protein (EFN-MM2A3) Summary

Product Description

Recombinant Mouse Ephrin-A3/EFNA3 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln23-Gly206.

Product Background

Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse Ephrin-A3/EFNA3 Protein (EFN-MM2A3) Specifications/Details

Protein
Ephrin-A3/EFNA3
Synonyms
Ephrin-A3; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EFNA3; EPLG3; LERK3
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Gln23-Gly206
Molecular Weight
The protein has a predicted MW of 47.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Product Tag
C-hFc (IgG1)

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Ephrin-A3 is greater than 95% as determined by SEC-HPLC. The purity of Mouse Ephrin-A3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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