Mouse EGF Protein (EGF-MM202)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 9.96 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asn977-Arg1029

Catalog: EGF-MM202

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Product Description Recombinant Mouse EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn977-Arg1029.

Product Background

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

Product Category

Recombinant Protein

Target

EGF

Gene/Protein ID

P01132

Species

Mouse

Exact Sequence

Asn977-Arg1029
Molecular Weight The protein has a predicted MW of 32.35 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 9.96 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 9.96 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.4 - 3 ng/mL.
The purity of Mouse EGF is greater than 95% as determined by SEC-HPLC.
Mouse EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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