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Mouse EGF Protein, Ultra Low Endotoxin (EGF-MM202-UL) Summary
Product Description |
Recombinant Mouse EGF Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Asn977-Arg1029. |
Product Background |
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Mouse EGF Protein, Ultra Low Endotoxin (EGF-MM202-UL) Specifications/Details
Protein |
EGF |
Synonyms |
HOMG4; EGF; URG |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Asn977-Arg1029 |
Molecular Weight |
The protein has a predicted MW of 32.35 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 3.98 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Mouse EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 3.98 nM as determined in SPR assay (Biacore T200). |
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Mouse EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.4 - 3 ng/mL. |
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The purity of Mouse EGF is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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