• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/ug.
  • Amino Acid Range: Ser49-Ile862
Catalog #ENP-MM101
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Mouse ENPP-2/Autotaxin Protein (ENP-MM101) Summary

Product Description

 Recombinant Mouse ENPP-2/Autotaxin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser49-Ile862.

Product Background

 Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse ENPP-2/Autotaxin Protein (ENP-MM101) Specifications/Details

Protein
 ENPP-2/Autotaxin
Synonyms
 PDNP2; ATX-X; NPP2; PD-IALPHA; ENPP-2; E-NPP 2; Autotaxin; LysoPLD; ATX;ENPP2
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Ser49-Ile862
Molecular Weight
 The protein has a predicted MW of 94.7 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/ug.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse ENPP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse ENPP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse ENPP-2 is greater than 95% as determined by SEC-HPLC. The purity of Mouse ENPP-2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...