• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fas Ligand, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse Fas, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA.
  • Amino Acid Range: Gln22-Arg169
Catalog #FAS-MM201
Price
Regular price $19800
Regular price Sale price $19800
/
Size
Avantor VWR Fisher Scientific

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Mouse Fas/TNFRSF6/CD95 Protein (FAS-MM201) Summary

Product Description

 Recombinant Mouse Fas/TNFRSF6/CD95 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln22-Arg169.

Product Background

 CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).

Mouse Fas/TNFRSF6/CD95 Protein (FAS-MM201) Specifications/Details

Protein
 Fas/TNFRSF6/CD95
Synonyms
 FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gln22-Arg169
Molecular Weight
 The protein has a predicted MW of 43.3 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Fas Ligand, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse Fas, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Fas Ligand, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse Fas, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA. Immobilized Human Fas Ligand, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse Fas, hFc Tag with the EC50 of 0.25ug/ml determined by ELISA.
Mouse Fas on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Fas on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fas is greater than 95% as determined by SEC-HPLC. The purity of Mouse Fas is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...