Mouse Fc gamma RI/CD64 Protein (FRI-MM164)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu25-Pro297

Catalog: FRI-MM164

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Product Description Recombinant Mouse Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Pro297.

Product Background

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma- RI (also known as CD64), Fc gamma- RII (CD32), and Fc gamma- RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

Product Category

Recombinant Protein

Target

Fc gamma RI/CD64
Synonyms CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI

Gene/Protein ID

P26151-1

Species

Mouse

Exact Sequence

Glu25-Pro297
Molecular Weight The protein has a predicted MW of 31.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Trastuzumab captured on CM5 Chip via Protein A can bind Mouse Fc gamma RI, His Tag with an affinity constant of 87.0 nM as determined in SPR assay (Biacore T200).
The purity of Mouse Fc gamma RI/CD64 is greater than 95% as determined by SEC-HPLC.
Mouse Fc gamma RI/CD64 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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