Mouse Fc gamma RI/CD64 Protein (FRI-MM164)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu25-Pro297
Catalog #FRI-MM164
Regular price $264.00
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Product Description

Recombinant Mouse Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Pro297.

Product Background

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma- RI (also known as CD64), Fc gamma- RII (CD32), and Fc gamma- RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

Product Category

Recombinant Protein / Antibody discovery / Fc receptors

Protein

Fc gamma RI/CD64

Synonyms

CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI; FcγRI; Fc γRI; Fcγ RI; Fc γ RI

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Glu25-Pro297

Molecular Weight

The protein has a predicted MW of 31.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Trastuzumab captured on CM5 Chip via Protein A can bind Mouse Fc gamma RI, His Tag with an affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). Trastuzumab captured on CM5 Chip via Protein A can bind Mouse Fc gamma RI, His Tag with an affinity constant of 87.0 nM as determined in SPR assay (Biacore T200).
Mouse Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RI is greater than 95% as determined by SEC-HPLC. The purity of Mouse Fc gamma RI is greater than 95% as determined by SEC-HPLC.

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