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Mouse Fc gamma RI/CD64 Protein (FRI-MM164) Summary
Product Description |
Recombinant Mouse Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Pro297. |
Product Background |
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma- RI (also known as CD64), Fc gamma- RII (CD32), and Fc gamma- RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Mouse Fc gamma RI/CD64 Protein (FRI-MM164) Specifications/Details
Protein |
Fc gamma RI/CD64 |
Synonyms |
CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI; FcγRI; Fc γRI; Fcγ RI; Fc γ RI |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu25-Pro297 |
Molecular Weight |
The protein has a predicted MW of 31.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Trastuzumab captured on CM5 Chip via Protein A can bind Mouse Fc gamma RI, His Tag with an affinity constant of 87.0 nM as determined in SPR assay (Biacore T200). |
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Mouse Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Trastuzumab, hFc Tag on ProA-Biosensor can bind Mouse Fc gamma RI, His Tag with an affinity constant of 56.70 nM as determined in BLI assay . |
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The purity of Mouse Fc gamma RI is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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