Name: Mouse Fc gamma RIIB/CD32b Protein (CDB-MM101)
Brand: KactusBio
SKU: CDB-MM101-100UG
Price: 264.0 USD
Availability: InStock

Mouse Fc gamma RIIB/CD32b Protein (CDB-MM101)

OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 uM as determined in SPR assay (Biacore T200).

Mouse Fc gamma RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded OKT3 on ProA-Biosensor can bind Mouse Fc gamma RIIB,His Tag with an affinity constant of 1.99 µM as determined in BLI assay (Gator).

The purity of Mouse Fc gamma RIIB is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: The affinity constant of 0.280 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Thr40-Arg217

Catalog #CDB-MM101

Size

100UG

500UG

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Mouse Fc gamma RIIB/CD32b Protein (CDB-MM101) Summary

Product Description	Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr40-Arg217.
Product Background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Mouse Fc gamma RIIB/CD32b Protein (CDB-MM101) Specifications/Details

Protein	Fc gamma RIIB/CD32b
Synonyms	Fc gamma RII; Fc gamma RIIB; CD32; CD32b; CDw32; FCG2; IGFR2; CD32A; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A; FcγRIIB; Fc γRIIB; Fcγ RIIB; Fc γ RIIB
Accession	NP_001070657.1
Species	Mouse
Biotinylated	no
Amino Acid Range	Thr40-Arg217
Molecular Weight	The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.280 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 uM as determined in SPR assay (Biacore T200).
	Mouse Fc gamma RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded OKT3 on ProA-Biosensor can bind Mouse Fc gamma RIIB,His Tag with an affinity constant of 1.99 µM as determined in BLI assay (Gator).
	The purity of Mouse Fc gamma RIIB is greater than 90% as determined by SEC-HPLC.