Mouse Fc gamma RIIB/CD32b Protein (CDB-MM101) Summary
Product Description |
Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr40-Arg217. |
Product Background |
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Mouse Fc gamma RIIB/CD32b Protein (CDB-MM101) Specifications/Details
Protein |
Fc gamma RIIB/CD32b |
Synonyms |
Fc gamma RII; Fc gamma RIIB; CD32; CD32b; CDw32; FCG2; IGFR2; CD32A; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A; FcγRIIB; Fc γRIIB; Fcγ RIIB; Fc γ RIIB |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Thr40-Arg217 |
Molecular Weight |
The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.280 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 uM as determined in SPR assay (Biacore T200). |
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Mouse Fc gamma RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded OKT3 on ProA-Biosensor can bind Mouse Fc gamma RIIB,His Tag with an affinity constant of 1.99 µM as determined in BLI assay (Gator). |
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The purity of Mouse Fc gamma RIIB is greater than 90% as determined by SEC-HPLC. |
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