Mouse Fc gamma RIIB/CD32b Protein (CDB-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.280 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr40-Arg217
Catalog #CDB-MM101
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Product Description
 Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr40-Arg217.
Product Background
 The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Product Category
 Recombinant Protein
Target
 Fc gamma RIIB/CD32b
Synonyms
 Fc gamma Receptor IIB/C; CD32; CD32b; CDw32; FCGR2B/C; CD32b/c; FcRII-b/c; FCG2; IGFR2; CD32A; Fc gamma RII; Fc gamma RIIB; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Thr40-Arg217
Molecular Weight
 The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.280 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 uM as determined in SPR assay (Biacore T200).
Mouse Fc gamma RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RIIB is greater than 95% as determined by SEC-HPLC.
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