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Mouse Fc gamma RIII/CD16 Protein (FGR-MM1R3) Summary
Product Description |
Recombinant Mouse Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu32-Thr215. |
Product Background |
Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Mouse Fc gamma RIII/CD16 Protein (FGR-MM1R3) Specifications/Details
Protein |
Fc gamma RIII/CD16 |
Synonyms |
IgG Fc receptor III; Fc-gamma RIII; FcRIII; FCGR3; CD16; CD16A; FCG3; FcgRIII; FCR-10; FCRIIIA; IGFR3; IMD20; FcγRIII; Fc γRIII; Fcγ RIII; Fc γ RIII |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu32-Thr215 |
Molecular Weight |
The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 2.34 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIII, His Tag with an affinity constant of 2.34 uM as determined in SPR assay (Biacore T200). |
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Mouse Fc gamma RIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse Fc gamma RIII is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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