Mouse Fc gamma RIII/CD16 Protein (FGR-MM1R3)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 2.34 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu32-Thr215

Catalog: FGR-MM1R3

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Product Description	Recombinant Mouse Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu32-Thr215.
Product Background	Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.
Product Category	Recombinant Protein

Target	Fc gamma RIII/CD16
Synonyms	IgG Fc receptor III; Fc-gamma RIII; FcRIII; FCGR3; CD16; CD16A; FCG3; FcgRIII; FCR-10; FCRIIIA; IGFR3; IMD20
Gene/Protein ID	Q5D5I8
Species	Mouse
Exact Sequence	Leu32-Thr215
Molecular Weight	The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 2.34 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIII, His Tag with an affinity constant of 2.34 uM as determined in SPR assay (Biacore T200).
	The purity of Mouse Fc gamma RIII is greater than 95% as determined by SEC-HPLC.
	Mouse Fc gamma RIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.