Mouse Fc gamma RIV/CD16-2 Protein (FCR-MM162)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly21-Gln203
Catalog #FCR-MM162
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Product Description
 Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly21-Gln203.
Product Background
 FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation.
Product Category
 Recombinant Protein
Target
 Fc gamma RIV/CD16-2
Synonyms
 CD16-2; FcgammaRIV; Fcgr4; FcγRIV
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gly21-Gln203
Molecular Weight
 The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200).
Mouse Fc gamma RIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RIV is greater than 95% as determined by SEC-HPLC.

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