• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 146 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly21-Gln203
Catalog #FCR-MM162
Price
Regular price $346.50
Regular price Sale price $346.50
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Size
Avantor VWR Fisher Scientific

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Mouse Fc gamma RIV/CD16-2 Protein (FCR-MM162) Summary

Product Description

 Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly21-Gln203.

Product Background

 FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation.

Product Category

 Recombinant Protein / Antibody discovery / Fc receptors

Mouse Fc gamma RIV/CD16-2 Protein (FCR-MM162) Specifications/Details

Protein
 Fc gamma RIV/CD16-2
Synonyms
 CD16-2; FcgammaRIV; Fcgr4; FcγRIV
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gly21-Gln203
Molecular Weight
 The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 146 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200). Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200).
Mouse Fc gamma RIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Fc gamma RIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse Fc gamma RIV, His Tag on Anti-His-Biosensor, can bind Anti-CD3 Antibody(OKT3) with an affinity constant of 0.21 µM as determined in BLI assay (Gator). Loaded Mouse Fc gamma RIV, His Tag on Anti-His-Biosensor, can bind Anti-CD3 Antibody(OKT3) with an affinity constant of 0.21 µM as determined in BLI assay (Gator).
The purity of Mouse Fc gamma RIV is greater than 95% as determined by SEC-HPLC. The purity of Mouse Fc gamma RIV is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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