Mouse Fc gamma RIV/CD16-2 Protein (FCR-MM162)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly21-Gln203

Catalog: FCR-MM162

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Product Description Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gly21-Gln203.
Product Category Recombinant Protein
Target Fc gamma RIV/CD16-2
Synonyms CD16-2; FcgammaRIV; Fcgr4
Gene/Protein ID A0A0B4J1G0-1
Species Mouse
Exact Sequence Gly21-Gln203
Molecular Weight The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200).
The purity of Mouse Fc gamma RIV/CD16-2 is greater than 95% as determined by SEC-HPLC.
Mouse Fc gamma RIV/CD16-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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