Product Description |
Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly21-Gln203. |
Product Background |
FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation. |
Product Category |
Recombinant Protein |
Protein |
Fc gamma RIV/CD16-2 |
Synonyms |
CD16-2; FcgammaRIV; Fcgr4; FcγRIV |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly21-Gln203 |
Molecular Weight |
The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200). |
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Mouse Fc gamma RIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse Fc gamma RIV is greater than 95% as determined by SEC-HPLC. |
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