Mouse Fc gamma RIV/CD16-2 Protein (FCR-MM162)
Catalog: FCR-MM162
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| Product Description | Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gly21-Gln203. |
| Product Category | Recombinant Protein |
| Target | Fc gamma RIV/CD16-2 |
| Synonyms | CD16-2; FcgammaRIV; Fcgr4 |
| Gene/Protein ID | A0A0B4J1G0-1 |
| Species | Mouse |
| Exact Sequence | Gly21-Gln203 |
| Molecular Weight | The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per _g by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200). |
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The purity of Mouse Fc gamma RIV/CD16-2 is greater than 95% as determined by SEC-HPLC. |
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Mouse Fc gamma RIV/CD16-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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