Name: Mouse FcRn Protein (FRN-MM101)
Brand: KactusBio
SKU: FRN-MM101-100UG
Price: 355.3 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog #FRN-MM101

Size

100UG

500UG

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Product Description	Recombinant Mouse FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ser22-Ser297 (FCGRT) and Ile21-Met119 (B2M).
Product Background	The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Protein	FcRn
Synonyms	FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Accession	Q61559(FCGRT)&P01887(B2M)
Species	Mouse
Biotinylated	no
Amino Acid Range	Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight	The protein has a predicted MW of 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result.
Activity	The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (Biacore T200) (QC Test).
	Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 0.18 µM as determined in SPR assay (Biacore T200).
	Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
	Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
	Mouse FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse FcRn is greater than 95% as determined by SEC-HPLC.