Mouse FcRn Protein (FRN-MM101)
Mouse FcRn Protein (FRN-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)
Catalog #FRN-MM101
Regular price $323.00
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Product Description
Recombinant Mouse FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Ser297 (FCGRT)&Ile21-Met119 (B2M).
Product Background
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
Product Category
Recombinant Protein
Target
FcRn
Synonyms
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight
The protein has a predicted MW of 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result.
Activity
The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (Biacore T200) (QC Test).
Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 54.53 nM as determined in SPR assay (Biacore T200).
Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
Mouse FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse FcRn is greater than 95% as determined by SEC-HPLC.

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