- Express System: HEK293
- Product Tag: C-His
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)
Product Description |
Recombinant Mouse FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Ser297 (FCGRT)&Ile21-Met119 (B2M). |
Product Background |
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
Product Category |
Recombinant Protein |
Target |
FcRn |
Synonyms |
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ser22-Ser297(FCGRT) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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