Mouse FcRn Protein (FRN-MM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog: FRN-MM101

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Product Description Recombinant Mouse FcRn Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ser22-Ser297 (FCGRT)&Ile21-Met119 (B2M).
Product Category Recombinant Protein
Target FcRn
Synonyms FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Gene/Protein ID Q61559(FCGRT)&P01887(B2M)
Species Mouse
Exact Sequence Ser22-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight The protein has a predicted MW of 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Tris-Bis PAGE result.
Activity The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (Biacore T200).
Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 54.53 nM as determined in SPR assay (Biacore T200).
Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
The purity of Mouse FcRn is greater than 95% as determined by SEC-HPLC.
Mouse FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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