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Mouse FcRn Protein, Ultra Low Endotoxin (FRN-MM101-UL) Summary
Product Description |
Recombinant Mouse FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ser22-Ser297 (FCGRT) and Ile21-Met119 (B2M). |
Product Background |
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Mouse FcRn Protein, Ultra Low Endotoxin (FRN-MM101-UL) Specifications/Details
Protein |
FcRn |
Synonyms |
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ser22-Ser297(FCGRT) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (Biacore T200). |
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Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 20.82 nM as determined in SPR assay (Biacore T200). |
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Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). |
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Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). |
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Mouse FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Mouse FcRn, His Tag on Anti-His-Biosensor can bind Trastuzumab, hFc Tag with an affinity constant of 40.00 nM as determined in BLI assay . |
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The purity of Mouse FcRn is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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