Mouse FLT3 Ligand Protein (FLT-MM13L)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse FLT3 Ligand, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 39.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly27-Gln189
Catalog #FLT-MM13L
Regular price $315.00
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Product Description
Recombinant Mouse FLT3 Ligand Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly27-Gln189.
Product Background
Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Product Category
Recombinant Protein
Target
FLT3 Ligand
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Gly27-Gln189
Molecular Weight
The protein has a predicted MW of 19.61 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse FLT3 Ligand, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 39.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse FLT3 Ligand, His Tag captured on CM5 Chip via anti-his antibody can bind Human FLT3, hFc Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse FLT3 Ligand, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 39.3ng/ml determined by ELISA (QC Test).
Mouse FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse FLT3 Ligand is greater than 95% as determined by SEC-HPLC.

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