Product Description |
Recombinant Mouse FLT3 Ligand Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly27-Gln189. |
Product Background |
Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. |
Product Category |
Recombinant Protein |
Target |
FLT3 Ligand |
Synonyms |
FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly27-Gln189 |
Molecular Weight |
The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
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Mouse FLT3 Ligand, hFc Tag captured on CM5 Chip via Protein A can bind Human FLT3, His Tag with an affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). |
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Mouse FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse FLT3 Ligand is greater than 95% as determined by SEC-HPLC. |
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