Mouse FLT3 Ligand Protein (FLT-MM23L)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly27-Gln189

Catalog: FLT-MM23L

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Product Description	Recombinant Mouse FLT3 Ligand Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly27-Gln189.
Product Background	Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Product Category	Recombinant Protein

Target	FLT3 Ligand
Synonyms	FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine
Gene/Protein ID	P49772-1
Species	Mouse
Exact Sequence	Gly27-Gln189
Molecular Weight	The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse FLT3 Ligand, hFc Tag captured on CM5 Chip via Protein A can bind Human FLT3, His Tag with an affinity constant of 13.81 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test).
	The purity of Mouse FLT3 Ligand is greater than 95% as determined by SEC-HPLC.
	Mouse FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.