Name: Mouse FLT3 Ligand Protein (FLT-MM23L)
Brand: KactusBio
SKU: FLT-MM23L-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly27-Gln189

Catalog #FLT-MM23L

Size

100UG

500UG

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Product Description	Recombinant Mouse FLT3 Ligand Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly27-Gln189.
Product Background	Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Product Category	Recombinant Protein / Antibody discovery

Protein	FLT3 Ligand
Synonyms	FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine
Accession	P49772-1
Species	Mouse
Biotinylated	no
Amino Acid Range	Gly27-Gln189
Molecular Weight	The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse FLT3 Ligand, hFc Tag captured on CM5 Chip via Protein A can bind Human FLT3, His Tag with an affinity constant of 13.81 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test).
	Mouse FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse FLT3 Ligand is greater than 95% as determined by SEC-HPLC.