Product Description |
Recombinant Mouse FOLR4/Juno Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Gly222. |
Product Background |
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
FOLR4/Juno |
Synonyms |
Folate receptor 4; FR-delta; FR delta; Izumo1r; Folbp3; Juno; FOLR4, LOC390243 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly20-Gly222 |
Molecular Weight |
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Mouse FOLR4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse FOLR4 is greater than 95% as determined by SEC-HPLC. |
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