• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly20-Gly222
Catalog #FOL-MM1R4
Price
Regular price $330.00
Regular price Sale price $330.00
/
Size
Avantor VWR Fisher Scientific

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Mouse FOLR4/Juno Protein (FOL-MM1R4) Summary

Product Description

 Recombinant Mouse FOLR4/Juno Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Gly222.

Product Background

 Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.

Mouse FOLR4/Juno Protein (FOL-MM1R4) Specifications/Details

Protein
 FOLR4/Juno
Synonyms
 Folate receptor 4; FR-delta; FR delta; Izumo1r; Folbp3; Juno; FOLR4, LOC390243
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gly20-Gly222
Molecular Weight
 The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse FOLR4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse FOLR4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse FOLR4 is greater than 95% as determined by SEC-HPLC. The purity of Mouse FOLR4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...