Mouse FSTL3 Protein (FTS-MM1L3)
Mouse FSTL3 Protein (FTS-MM1L3)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Activin A, No Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 53.09 pM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val24-Val256
Catalog #FTS-MM1L3
Regular price $270.00
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Product Description
Recombinant Mouse FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Val256.
Product Background
Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
Product Category
Recombinant Protein
Target
FSTL3
Synonyms
FLRG; FLRGFSRP; Follistatin-like 3; FSTL3
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Val24-Val256
Molecular Weight
The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Activin A, No Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 53.09 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse FSTL3, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A, No tag-with an affinity constant of 53.09 pM as determined in SPR assay (Biacore T200).
Immobilized Human Activin A, No Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
Mouse FSTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.
The purity of Mouse FSTL3 is greater than 95% as determined by SEC-HPLC.

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