Product Description |
Recombinant Mouse FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Val256. |
Product Background |
Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD. |
Product Category |
Recombinant Protein |
Target |
FSTL3 |
Synonyms |
FLRG; FLRGFSRP; Follistatin-like 3; FSTL3 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Val24-Val256 |
Molecular Weight |
The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Activin A, No Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 53.09 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse FSTL3, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A, No tag-with an affinity constant of 53.09 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human Activin A, No Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). |
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|
Mouse FSTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A. |
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The purity of Mouse FSTL3 is greater than 95% as determined by SEC-HPLC. |
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