Mouse G-CSF R/CD114-Protein (GSR-MM101)
Mouse G-CSF R/CD114-Protein (GSR-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Cys26-Asp626
Catalog #GSR-MM101
Regular price $270.00
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Product Description
Recombinant Mouse G-CSF R/CD114-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys26-Asp626.
Product Background
The Granulocyte Colony-Stimulating Factor (G-CSF) receptor, a member of the hematopoietin cytokine receptor superfamily, functions as a homodimer and requires the recruitment of cytosolic protein tyrosine kinases (PTKs) to transduce its signal.
Product Category
Recombinant Protein
Target
G-CSF R/CD114
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Cys26-Asp626
Molecular Weight
The protein has a predicted MW of 68.47 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA.
Mouse G-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse G-CSF R is greater than 95% as determined by SEC-HPLC.

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