Mouse G-CSF R/CD114-Protein (GSR-MM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Cys26-Asp626

Catalog: GSR-MM101

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Product Description	Recombinant Mouse G-CSF R/CD114-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys26-Asp626.
Product Background	The Granulocyte Colony-Stimulating Factor (G-CSF) receptor, a member of the hematopoietin cytokine receptor superfamily, functions as a homodimer and requires the recruitment of cytosolic protein tyrosine kinases (PTKs) to transduce its signal.
Product Category	Recombinant Protein

Target	G-CSF R/CD114
Gene/Protein ID	P40223
Species	Mouse
Exact Sequence	Cys26-Asp626
Molecular Weight	The protein has a predicted MW of 68.47 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA.
	The purity of Mouse G-CSF R is greater than 95% as determined by SEC-HPLC.
	Mouse G-CSF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.