Product Description |
Recombinant Mouse G-CSF R/CD114-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys26-Asp626. |
Product Background |
The Granulocyte Colony-Stimulating Factor (G-CSF) receptor, a member of the hematopoietin cytokine receptor superfamily, functions as a homodimer and requires the recruitment of cytosolic protein tyrosine kinases (PTKs) to transduce its signal. |
Product Category |
Recombinant Protein |
Target |
G-CSF R/CD114 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Cys26-Asp626 |
Molecular Weight |
The protein has a predicted MW of 68.47 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Mouse G-CSF R, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA. |
|
|
Mouse G-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse G-CSF R is greater than 95% as determined by SEC-HPLC. |
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