Mouse GFRA3 Protein (GFR-MM1A3)
Mouse GFRA3 Protein (GFR-MM1A3)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 32.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asn29-Arg379
Catalog #GFR-MM1A3
Regular price $300.00
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Product Description

Recombinant Mouse GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn29-Arg379.

Product Background

GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.

Product Category

Recombinant Protein

Protein

GFRA3

Synonyms

GDNFR-alpha-3; GFR-alpha-3; GFRA3; GFR alpha3; GPI-linked receptor

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Asn29-Arg379

Molecular Weight

The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 47-52 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 32.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 32.2ng/ml determined by ELISA. Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 32.2ng/ml determined by ELISA.
Mouse GFRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse GFRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GFRA3 is greater than 95% as determined by SEC-HPLC. The purity of Mouse GFRA3 is greater than 95% as determined by SEC-HPLC.

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