Mouse GFRAL/GFR alpha-like Protein (GFL-MM401)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Mouse GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln20-Glu350

Catalog: GFL-MM401

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Product Description Recombinant Mouse GFRAL/GFR alpha-like Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.; It contains Gln20-Glu350.
Product Category Recombinant Protein
Target GFRAL/GFR alpha-like
Synonyms GFR alpha-like; GFRAL; GRAL; C6orf144
Gene/Protein ID Q6SJE0-1
Species Mouse
Exact Sequence Gln20-Glu350
Molecular Weight The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse GFRAL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC.
Mouse GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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