Mouse GFRAL/GFR alpha-like Protein (GFL-MM401)
Catalog: GFL-MM401
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| Product Description | Recombinant Mouse GFRAL/GFR alpha-like Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Gln20-Glu350. |
| Product Category | Recombinant Protein |
| Target | GFRAL/GFR alpha-like |
| Synonyms | GFR alpha-like; GFRAL; GRAL; C6orf144 |
| Gene/Protein ID | Q6SJE0-1 |
| Species | Mouse |
| Exact Sequence | Gln20-Glu350 |
| Molecular Weight | The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. |
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The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC. |
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Mouse GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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