Mouse GFRAL/GFR alpha-like Protein (GFL-MM401)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Mouse GFRAL, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln20-Glu350
Catalog #GFL-MM401
Regular price $315.00
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Product Description
Recombinant Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.; It contains Gln20-Glu350.
Product Background
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Product Category
Recombinant Protein
Target
GFRAL/GFR alpha-like
Synonyms
GFR alpha-like; GFRAL; GRAL; C6orf144
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Gln20-Glu350
Molecular Weight
The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse GFRAL, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse GFRAL, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test).
Mouse GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC.

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