Mouse GM-CSF Protein (GCF-MM201)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala18-Lys141

Catalog: GCF-MM201

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Product Description Recombinant Mouse GM-CSF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala18-Lys141.

Product Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.

Product Category

Recombinant Protein

Target

GM-CSF

Gene/Protein ID

P01587

Species

Mouse

Exact Sequence

Ala18-Lys141
Molecular Weight The protein has a predicted MW of 39.64 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse GM-CSF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.
The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC.
Mouse GM-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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