Product Description |
Recombinant Mouse GM-CSF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala18-Lys141. |
Product Background |
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. |
Product Category |
Recombinant Protein |
Protein |
GM-CSF |
Synonyms |
CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala18-Lys141 |
Molecular Weight |
The protein has a predicted MW of 39.64 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). |
|
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Mouse GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC. |
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