• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala18-Lys141
Catalog #GCF-MM201
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Mouse GM-CSF Protein (GCF-MM201) Summary

Product Description

 Recombinant Mouse GM-CSF Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala18-Lys141.

Product Background

 Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.

Mouse GM-CSF Protein (GCF-MM201) Specifications/Details

Protein
 GM-CSF
Synonyms
 CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897;Colony stimulating factor-2; Colony stimulating factor 2
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Ala18-Lys141
Molecular Weight
 The protein has a predicted MW of 39.64 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Mouse GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC. The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...