Name: Mouse GM-CSF Protein (GCF-MM201)
Brand: KactusBio
SKU: GCF-MM201-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala18-Lys141

Catalog #GCF-MM201

Size

100UG

500UG

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Product Description	Recombinant Mouse GM-CSF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala18-Lys141.
Product Background	Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	GM-CSF
Synonyms	CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897
Accession	P01587
Species	Mouse
Biotinylated	no
Amino Acid Range	Ala18-Lys141
Molecular Weight	The protein has a predicted MW of 39.64 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
	Mouse GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse GM-CSF is greater than 95% as determined by SEC-HPLC.