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Mouse GM-CSF R alpha Protein, Ultra Low Endotoxin (CSF-MM12R-UL) Summary
Product Description |
Recombinant Mouse GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Pro327. |
Product Background |
The receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF) is composed of an alpha and beta subunit, which together form the high-affinity receptor. The alpha subunit by itself binds ligand at low affinity, whereas the isolated beta subunit does not bind GM-CSF. It is generally believed that the high-affinity receptor is responsible for the multiple functions of GM-CSF and that the isolated alpha subunit (GMR alpha) does not transduce a signal. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse GM-CSF R alpha Protein, Ultra Low Endotoxin (CSF-MM12R-UL) Specifications/Details
Protein |
GM-CSF R alpha |
Synonyms |
GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu30-Pro327 |
Molecular Weight |
The protein has a predicted MW of 33.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). The affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse GM-CSF R alpha, His Tag captured on CM5 Chip via Anti-his antibody can bind Mouse GM-CSF, No Tag with an affinity constant of 6.32 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse GM-CSF R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). |
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Mouse GM-CSF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse GM-CSF R alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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