Mouse GPA33/A33 Protein (GPA-MM133) Summary
Product Description |
Recombinant Mouse GPA33/A33 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu22-Ile235. |
Product Background |
Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse GPA33/A33 Protein (GPA-MM133) Specifications/Details
Protein |
GPA33/A33 |
Synonyms |
GPA33; A33; Cell surface A33 antigen; Glycoprotein A33; mA33 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu22-Ile235 |
Molecular Weight |
The protein has a predicted MW of 24.91 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Mouse GPA33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse GPA33 is greater than 95% as determined by SEC-HPLC. |
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