Name: Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453)
Brand: KactusBio
SKU: MHC-MM453-100UG
Price: 341.0 USD
Availability: InStock

Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453)

Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide

Catalog #MHC-MM453

Size

100UG

500UG

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Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453) Summary

Product Description	Recombinant Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297 (H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide.
Product Background	Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

H-2K(b)&B2M&OVA (SIINFEKL) Specifications

Protein	H-2K(b)&B2M&OVA (SIINFEKL)
Synonyms	H-2K; OVA; MHC; H2-K1;
MHC Form	Monomer
Allele	H-2K (b)
Accession	P01901(H-2K(b))&P01887(B2M)&SIINFEKL
Species	Mouse
Biotinylated	no
Amino Acid Range	His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide
Molecular Weight	The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer is greater than 95% as determined by SEC-HPLC.