• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Val309(H2-Db), Ile21-Met119(B2M) and KAVYNFATC peptide
Catalog #MHC-ME402
Price
Regular price $34100
Regular price Sale price $34100
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Size
Avantor VWR Fisher Scientific

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Mouse H2-Db&B2M&LCMV gp33-41 (KAVYNFATC) Monomer Protein (MHC-ME402) Summary

Product Description

 Recombinant Mouse H2-Db&B2M&LCMV gp33-41 (KAVYNFATC) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Val309 (H2-Db), Ile21-Met119 (B2M) and KAVYNFATC peptide.

Product Background

 LCMV gp33-41 (KAVYNFATC) is an H-2Db-restricted immunodominant epitope derived from the viral envelope glycoprotein (GP) of the Lymphocytic Choriomeningitis Virus. It serves as a critical target for CD8+ T cell responses and is widely utilized in immunological models, notably using P14 TCR transgenic mice, to study T cell activation and viral clearance.

H2-Db&B2M&LCMV gp33-41 (KAVYNFATC) Specifications

Protein
 H2-Db&B2M&LCMV gp33-41 (KAVYNFATC)
MHC Form
 Monomer
Allele
 H2-Db
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gly25-Val309(H2-Db), Ile21-Met119(B2M) and KAVYNFATC peptide
Molecular Weight
 The protein has a predicted MW of 35.93 kDa (H2-Db) and 11.64 kDa (B2M) same as Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse H2-Db&B2M&LCMV gp33-41 (KAVYNFATC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse H2-Db&B2M&LCMV gp33-41 (KAVYNFATC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse H2-Db&B2M&LCMV gp33-41 (KAVYNFATC) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Mouse H2-Db&B2M&LCMV gp33-41 (KAVYNFATC) Monomer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...