Product Description |
Recombinant Mouse HGFA Protein (pro form) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Ser653. |
Product Background |
Hepatocyte growth factor activator (HGFA) is a serine protease initially identified as a potent activator of hepatocyte growth factor/scatter factor. Hepatocyte growth factor/scatter factor is known to be critically involved in tissue morphogenesis, regeneration, and tumor progression, via its receptor, MET.In vivo, HGFA also activates macrophage-stimulating protein, which has roles in macrophage recruitment and inflammatory processes, cellular survival and wound healing through its receptor, RON. |
Product Category |
Recombinant Protein |
Target |
HGFA |
Synonyms |
HGF activator; HGFA; Hgfac; MGC138395; MGC138397 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln35-Ser653 |
Molecular Weight |
The protein has a predicted MW of 67.9 kDa. Due to glycosylation, the protein (pro form) migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl, 2mM CaCl2 (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse HGFA (pro form) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse HGFA (pro form) is greater than 95% as determined by SEC-HPLC. |
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