Product Description |
Recombinant Mouse&Human Chimeric Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed from HEK293 with His tag at the C-terminus of ITGAV.It contains Phe31-Val988 (ITGAV) acidic tail and Gly22-Asn707 (ITGB6) basic tail. |
Product Background |
ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer |
Synonyms |
αVβ6; Integrin alpha V beta 6; ITGAV&ITGB6;Integrin alpha V β 6 |
Accession |
|
Species |
Mouse&Human |
Biotinylated |
no |
Amino Acid Range |
Phe31-Val988(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail |
Molecular Weight |
The protein has a predicted MW of 131.48 kDa (ITGAV) and 79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 120-160 kDa (ITGAV) and 90-120 kDa (ITGB6) based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse&Human Chimeric ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His-Avi Tag with the EC50 of 36.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Mouse&Human Chimeric ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His-Avi Tag with the EC50 of 36.8ng/ml determined by ELISA. |
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Immobilized Human&Mouse Chimeric ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1), His Tag with the EC50 of 78.4ng/ml determined by ELISA. |
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Immobilized Mouse&Human Chimeric ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB6 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. |
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Mouse&Human Chimeric ITGAV&ITGB6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-ITGB6 Ab., hFc-Avi Tag on ProA-Biosensor can bind Human&Mouse Chimeric ITGAV&ITGB6, His Tag with an affinity constant of 76.20 nM as determined in BLI assay (Gator® Prime). |
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The purity of Mouse&Human Chimeric ITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC. |
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