• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Glu21-Leu144
Catalog #ICO-MM201
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Mouse ICOS/CD278 Protein (ICO-MM201) Summary

Product Description

 Recombinant Mouse ICOS/CD278 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu21-Leu144.

Product Background

 Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

Mouse ICOS/CD278 Protein (ICO-MM201) Specifications/Details

Protein
 ICOS/CD278
Synonyms
 AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850;CRP1
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Glu21-Leu144
Molecular Weight
 The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. The affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200). Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA. Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 91.2ng/ml determined by ELISA.
Mouse ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse ICOS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse ICOS, hFc Tag on ProA-Biosensor can bind Rat B7-H2, His Tag with an affinity constant of 0.40 µM as determined in BLI assay. Loaded Mouse ICOS, hFc Tag on ProA-Biosensor can bind Rat B7-H2, His Tag with an affinity constant of 0.40 µM as determined in BLI assay.
Other Available Proteins:
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