Mouse IgG1 Fc Protein (MFC-MM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val98-Lys324
Catalog #MFC-MM001
Regular price $90.00
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Product Description
Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag.It contains Val98-Lys324.
Product Background
Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.
Product Category
Recombinant Protein
Target
IgG1 Fc
Synonyms
IgG1; Igh-4; RGD1359539; VH7183
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Val98-Lys324
Molecular Weight
The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
No Tag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
Mouse IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IgG1 Fc is greater than 95% as determined by SEC-HPLC.

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