Product Description |
Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag.It contains Val98-Lys324. |
Product Background |
Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Protein |
IgG1 Fc |
Synonyms |
IgG1; Igh-4; RGD1359539; VH7183 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Val98-Lys324 |
Molecular Weight |
The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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|
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). |
|
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Mouse IgG1 Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse IgG1 Fc is greater than 90% as determined by SEC-HPLC. |
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