Mouse IgG1 Fc Protein (MFC-MM001)
Catalog: MFC-MM001
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| Product Description | Recombinant Mouse IgG1 Fc Protein is expressed from Expi293 without tag.It contains Val98-Lys324. |
| Product Category | Recombinant Protein |
| Target | IgG1 Fc |
| Synonyms | IgG1; Igh-4; RGD1359539; VH7183 |
| Gene/Protein ID | P01868 |
| Species | Mouse |
| Exact Sequence | Val98-Lys324 |
| Molecular Weight | The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 34-36 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | No Tag |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). |
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The purity of Mouse IgG1 Fc is greater than 95% as determined by SEC-HPLC. |
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Mouse IgG1 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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