Product Description |
Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330. |
Product Background |
Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life. |
Product Category |
Recombinant Protein |
Target |
IgG2a Fc |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu98-Lys330 |
Molecular Weight |
The protein has a predicted MW of 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). |
|
|
Mouse IgG2a Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC. |
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