Mouse IgG2a Fc Protein (IGG-MM02A)

  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu98-Lys330

Catalog: IGG-MM02A

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Product Description Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330.

Product Background

Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.

Product Category

Recombinant Protein

Target

IgG2a Fc
Accession

Species

Mouse

Amino Acid Range

Glu98-Lys330
Molecular Weight The protein has a predicted MW of 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

No Tag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC.
Mouse IgG2a Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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