- Express System: HEK293
- Product Tag: No Tag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
- Amino Acid Range: Glu98-Lys330
Product Description |
Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330. |
Product Background |
Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
IgG2a Fc |
Synonyms |
IgG2a; IgG2a-Fc; Igh-1; Igh 1; Immunoglobulin G2A Fc; G2A Fc |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu98-Lys330 |
Molecular Weight |
The protein has a predicted MW of 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 2.72 nM as determined in SPR assay (Biacore T200). |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.