Mouse IL-1 alpha/IL-1A Protein (IL1-MM10A)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 51.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser115-Ser270
Catalog #IL1-MM10A
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Product Description
 Recombinant Mouse IL-1 alpha/IL-1A Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser115-Ser270.
Product Background
 The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.
Product Category
 Recombinant Protein
Target
 IL-1 alpha/IL-1A
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Ser115-Ser270
Molecular Weight
 The protein has a predicted MW of 19.08 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 51.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse IL-1R1, His Tag immobilized on CM5 Chip can bind Mouse IL-1 alpha, His Tag with an affinity constant of 51.22 nM as determined in SPR assay (Biacore T200).
Mouse IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IL-1 alpha is greater than 95% as determined by SEC-HPLC.

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