Mouse IL-12 Protein (IL1-MM112) Summary
Product Description |
Recombinant Mouse IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus of IL-12A.It contains Arg23-Ala215(IL-12A) and Met23-Ser335(IL-12B). |
Product Background |
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse IL-12 Protein (IL1-MM112) Specifications/Details
Protein |
IL-12 |
Synonyms |
CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Arg23-Ala215(IL-12A) & Met23-Ser335(IL-12B) |
Molecular Weight |
The protein has a predicted MW of 22.80 kDa(IL-12A)&35.79 kDa(IL-12B). Due to glycosylation, the protein migrates to 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Mouse IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.5 - 2 ng/mL. |
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The purity of Mouse IL-12 is greater than 95% as determined by SEC-HPLC. |
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