Mouse IL-12 Protein (IL1-MM112)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Arg23-Ala215(IL-12A) & Met23-Ser335(IL-12B)

Catalog: IL1-MM112


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Product Description Recombinant Mouse IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg23-Ala215(IL-12A)&Met23-Ser335(IL-12B).

Product Background

Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Product Category

Recombinant Protein





Amino Acid Range

Arg23-Ala215(IL-12A) & Met23-Ser335(IL-12B)
Molecular Weight The protein has a predicted MW of 22.80 kDa(IL-12A)&35.79 kDa(IL-12B). Due to glycosylation, the protein migrates to 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) based on Tris-Bis PAGE result.

Product Tag


Express System

Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.5 - 2 ng/mL.
The purity of Mouse IL-12 is greater than 95% as determined by SEC-HPLC.
Mouse IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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